This project uses chromatography to separate and analyze different pigments found in plants. Students will learn how to identify these pigments and understand the science behind their separation, engaging with the beauty of nature.
Different pigments from plants will travel different distances on chromatography paper based on their solubility in the solvent used.
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Prepare plant material by crushing it to extract pigments.
Draw a baseline on the chromatography paper with a pencil.
Place a small spot of the extracted pigment on the baseline.
Fill the beaker with solvent to a shallow depth.
Carefully place the chromatography paper into the solvent, ensuring the spot isn't submerged.
Watch as the pigments separate up the paper.
Once the solvent has traveled, remove the paper and mark the solvent front.
Analyze the distance traveled by each pigment and identify them.
You will observe distinct bands of color representing different pigments, demonstrating how chromatography effectively separates substances based on their properties.
Type of plant material used
Distance traveled by pigments
Type of solvent, Amount of solvent, Size of pigment spot
Use safety goggles while handling solvents and scissors to avoid injuries.
Display your chromatography paper with clear visuals.
Explain the science behind chromatography and its applications.
Encourage audience participation by discussing pigment identification.
Include photos of the process for clarity.
