Science Fair Projects World

The Role of Enzymes in Food Digestion




Explore the role of enzymes in the digestion process by testing the effect of different digestive enzymes on a food substrate.


  • Test tubes
  • Milk (substrate)
  • Enzymes (e.g., amylase, protease, lipase)
  • Dropper
  • Water bath or thermometer
  • Stopwatch
  • Notebook for observations


  1. Label the test tubes for each enzyme type.
  2. Pour equal amounts of milk into each test tube.
  3. Add a few drops of amylase to one test tube, protease to another, and lipase to the third.
  4. Place the test tubes in a water bath set to a specific temperature (e.g., 37°C) or monitor and maintain the temperature manually.
  5. Use a stopwatch to time the reaction for each enzyme.
  6. Observe and note any visible changes in the milk, such as curdling or separation.
  7. Record the results in a notebook, including the time taken for digestion and the extent of the changes observed.
  8. Analyze the results to understand the effectiveness of each enzyme in breaking down milk components.